Dactylorhiza sambucina Elder Flowered Orchid (Mixture of Red and Yellow Bulgarian Ecotypes)


General Information

The seed of this species was purchased form Chris Greenwell from Bulgaria. Flower color comes in two varieties red and white. The species is found in dry  meadows on siliceous or calcareous soils.



Plating Media: F522 medium + 2 uM MetaTopolin, 4, 1 cm3 cubes Russet potato  sliced in quarters  25 mins. 0.525% Chlorox bleaching (no charcoal).  Seeds were plated 11/24/11 on 150 mm petri dishes containing 100 ml of media.  Plates were sealed with parafilm and incubated in the dark at room temperature ( approx. 23-26 o C).  Plates were examined on Jan. 29, 2012 and good germination was observed. There were some brown protocorms.


Number of Days between Plating and Replating:  approximately 67 days



Replating Media:  F522 medium + 20 ml/l pineapple juice + 1, 1 cm3 cube Russet potato sliced in quarters + 20 ml/l pineapple juice + 0.5g/l charcoal.  Replating was done in  wide mouth pint jars containing 200 ml of media on Jan. 29, 2012 and replates were kept in the dark.

Replates were examined on 8/22/2012 after 217 days. The plants showed good shoot growth with shoots from 1/2 inch to 1 inches with most shoots about 1/2  inches.  The protocorms often developed multiple short shoots. The shoots were stout and not spindly.  Good root development was also observed but the roots had difficulty penetrating the media. Probably need to reduce the amount of agar for better root penetration. The roots looked healthy and showed no signs of phenolic compounds (this would be hard to see with the presence of charcoal--- but the roots looked excellent). Jars of seedlings were placed in the frig in darkness on Aug. 22, 2012 to start vernalization.




Number of Days between Replating and Vernalization: 217 days


Number of Days between start and end of Vernalization: ---


Date to Initiate Plating: 11/24



Date to Replate:  Feb. . 29



Date to start to Vernalize: August. 22 (217 days after replating)



Results after Vernalization:


Results After Outplanting in Media:



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