Dactylorhiza saccifera Sack-carrying Dactylorhiza (Bulgaria ecotype)


General Information

The seed of this species was purchased form Chris Greenwell from Bulgaria. Flower color is pink. The labellum which has three lobes, the middle one the largest typically has darker purple loop markings. Leaves are not spotted. Habitat is unknown.



Plating Media: R medium + 2 uM 2 ip + 4, 1 cm3 cubes Russet potato  sliced in quarters.  25 mins. 0.525% Chlorox bleaching (no charcoal).  Seeds were plated 10/9/2011 on 150 mm petri dishes containing 100 ml of media.  Plates were sealed with parafilm and incubated in the dark at room temperature ( approx. 23-26 o C).  Plates were examined on Nov. 25, 2011 and excellent germination was noted. The protocorms are large and at the round top stage.


Number of Days between Plating and Replating:  approximately 46 days



Replating Media:  F522 medium (agar included) + 20 ml/l pineapple juice + 1, 1 cm3 cube Russet  potato  sliced in quarters + 20 ml/l pineapple juice + 0.5g/l charcoal.  Replating was done in  wide mouth pint jars containing 200 ml of media on Jan. 3, 2012. Replates were kept in the dark.  Replates were examined on 8/23/2012 after 218 days. The plants showed moderate shoot growth with shoots around 1/2 inches.  A few shoots were larger..  The shoots were stout and not spindly.  In most cases there were multiple shoots emanating from the seedling probably the effect of using a cytokinin to stimulate germination. Root development was poor with few roots penetrating the media--- only larger shoots had larger roots. Jars of seedlings were placed in the frig on Aug. 23, 2012 to start vernalization. (Note: results comparable to the above were obtained with several other replate media namely:  P668, 3/4 strength P668, and R media each supplemented with 1 potato and 20 ml/l pineapple.)




Number of Days between Replating and Vernalization: 218 days


Number of Days between start and end of Vernalization: ---


Date to Initiate Plating: 10/9



Date to Replate:  Jan. 3



Date to start to Vernalize: August. 239 (218 days after replating)



Results after Vernalization:


Results After Outplanting in Media:



Dactylorhiza saccifera 2.jpg                 Dactylorhiza saccifera 3.jpg

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