The seed of this species was donated by Russ Graham in Oregon in 2008. Someone in the area gave it to him. This species is found in wet meadows and marshes. The flowers range in color from pink to purple and possess distinctive dark purple loops and lines on the labelleum.
Plating Media: R medium + 2 uM 2ip + 4, 1 cm3 cubes Russet potato sliced in quarters 25 mins. 0.525% Chlorox bleaching (no charcoal). Seeds were plated 10/8/2011 on 150 mm petri dishes containing 100 ml of media. Plates were sealed with parafilm and incubated in the dark at room temperature (approx. 23-26 o C). Plates were examined on Dec. 21, 2011 and good germination was observed.
Number of Days between Plating and Replating: approximately 74 days
Replating Media: R medium +1, 1 cm3 cube Russet potato sliced in quarters + 20 ml/l pineapple juice (pH 5.7) + 0.5g/l charcoal + 3.5 g/l agargellan. Replating was done in wide mouth pint jars containing 200 ml of media on Dec. 21,2011 and replates were kept in the dark. Replates were examined on 8/23/2012 after 245 days. The plants showed good shoot growth with shoots from 1/2 inch to 1 inches with most shoots about 1/2 inches. Some protocorms often developed multiple short shoots which were shorter than seedlings which produced a single shoot. In general the shoots were stout and not spindly. Good root development was also observed in the larger shooted plants. Probably need to reduce the amount of agar for better root penetration. The roots looked healthy and showed no signs of phenolic compounds (this would be hard to see with the presence of charcoal--- but the roots looked excellent). Jars of seedlings were placed in the frig in darkness on Aug. 23, 2012 to start vernalization.
Number of Days between Replating and Vernalization: 245 days
Number of Days between start and end of Vernalization: ---
Date to Initiate Plating: 10/8
Date to Replate: Dec. 21
Date to start to Vernalize: August. 23 (245 days after replating)
Results after Vernalization:
Results After Outplanting in Media: