The seed of this species was purchased form Chris Greenwell from Bulgaria. Flower color is variable but generally pink grading to light purple. The labellum typically has darker purple loop markings. Leaves are not spotted. The species is found in wet meadows on base rich soils.
Plating Media: F522 medium + 2 uM MetaTopolin, 4, 1 cm3 cubes Russet potato sliced in quarters 25 mins. 0.525% Chlorox bleaching (no charcoal). Seeds were plated 11/24/11 on 150 mm petri dishes containing 100 ml of media. Plates were sealed with parafilm and incubated in the dark at room temperature ( approx. 23-26 o C). Plates were examined on Jan. 18, 2012 and good germination was observed. The protocorms are large and at the round top stage. Not many dead protocorms.
Number of Days between Plating and Replating: approximately 55 days
Replating Media: F522 medium + 20 ml/l pineapple juice + 1, 1 cm3 cube Russet potato sliced in quarters + 20 ml/l pineapple juice + 0.5g/l charcoal. Replating was done in wide mouth pint jars containing 200 ml of media on Jan. 18, 2012. Replates were kept in the dark.
Replates were examined on 8/19/2012 after 214 days. The plants showed excellent shoot growth with shoots from 1/2 inch to 1.5 inches with most shoots about 1 inch. The shoots were stout and not spindly. Good root development was also observed with numerous long roots. The roots looked healthy and no signs of phenolic compounds (this would be hard to see with the presence of charcoal--- but the roots looked excellent). Jars of seedlings were placed in the frig on Aug. 19, 2012 to start vernalization.
Number of Days between Replating and Vernalization: 214 days
Number of Days between start and end of Vernalization: ---
Date to Initiate Plating: 11/24
Date to Replate: Jan. 18
Date to start to Vernalize: August. 19 (214 days after replating)
Results after Vernalization:
Results After Outplanting in Media: