Cypripedium reginae


Plating Media: R medium + 2 uM metatopolin +  4, 1cm3 cubes Russet potatoes. 60 mins. bleaching with 0.525% Chlorox bleach


Replating Media:  R medium + 1, 1cm3 cube Russet potato



Number of Days between Plating and Replating:  65 days



Number of Days between Replating and Vernalization: 253 days



Number of Days between start and end of Vernalization: 120 days (4 months)




Date to Initiate Plating: Jan. 15



Date to Replate:  March 23



Date to start to Vernalize:  Dec. 1






Seedlings original plated on Nov. 10,2009, replated on  Jan. 14, 2010 and began vernalization on Sept. 27, 2010  have grown well with shoots about 1/2 to 3/4 inches tall with 2-4 roots 3 inches long.  As indicated above need to begin plating much earlier to avoid having the seedlings in vernalization for 6 months. This promotes fungused seedlings. The plating and replating media works very well. Stick with it. The seeds germinate very quickly.

Return to Orchid Propagation Protocols