Cypripedium parviflorum (from Garden - originally from seed from Minnesota provided by Bill Steele)


Plating Media: R medium + 2 uM metatopolin + 4, 1cm3 cubes Russet potatoes. 45 mins. bleaching with 0.525% Chlorox bleach.



Replating Media:  R medium + 1, 1cm3 cube Russet potato



Number of Days between Plating and Replating:  125  days



Number of Days between Replating and Vernalization:  5 months




Number of Days between start and end of Vernalization: 120 days (4 months)




Date to Initiate Plating:March 1



Date to Replate: June 1



 Date to start to Vernalize: Nov. 1





Seedlings were plated on 2/1/11, replated on 05-10-2011, and started vernalization on 10-09-11.  Seedlings had 1 inch shoots and 3-4 roots 1-2 inches long.  Started to vernalize earlier than normal because 11 jars had developed fungus.  8 jars remain to be un-flasked.  Should note that these seedlings did better than pubescens using the 2 uM MT.

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