Plating Media: R medium + 2 uM metatopolin + 4, 1cm3 cubes Russet potatoes. 45 mins. bleaching with 0.525% Chlorox bleach.
Replating Media: R medium + 1, 1cm3 cube Russet potato
Number of Days between Plating and Replating: 125 days
Number of Days between Replating and Vernalization: 5 months
Number of Days between start and end of Vernalization: 120 days (4 months)
Date to Initiate Plating:March 1
Date to Replate: June 1
Date to start to Vernalize: Nov. 1
Seedlings were plated on 2/1/11, replated on 05-10-2011, and started vernalization on 10-09-11. Seedlings had 1 inch shoots and 3-4 roots 1-2 inches long. Started to vernalize earlier than normal because 11 jars had developed fungus. 8 jars remain to be un-flasked. Should note that these seedlings did better than pubescens using the 2 uM MT.