Cypripedium guttatum

Plating Media: R medium + 2 uM metatopolin + 4, 1 cm3 cubes Russet potato. 25 mins. bleaching with 0.525% chlorox.

Replating Media: R medium + 1, 1 cm3 cube Russet potato

Number of Days between Plating and Replating: 112 days

Number of Days between Replating and Vernalization: 193 days

Number of Day between the start and end of Vernalization: 120 days (4 months)


Date to Initiate Plating: Jan. 30

Date to Replate: May 21

Date to start to Vernalize: Dec. 1




Seedlings plated on 11-10-09, replated on 03-01-2010, and started vernalization on 9-10-2010. Seedlings had 1/2 inch shoots and 3-8 roots 2-3 inches long. Need to start plating on Jan. 30 to avoid the long vernalization time. Overall, the protocol produced good results.

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